Principle

Status

A plastic support coated with specific mouse monoclonal anti-human CD146 F(ab')2 fragments (Reagent 1) binds to the sCD146 to be measured. Next the mouse monoclonal antibody anti-CD146 coupled with peroxidase (Reagent 6) binds to the remaining free antigenic determinants of the CD146. The bound enzyme peroxidase is then revealed by its activity in a predetermined time on the OPD substrate (Reagent 3a) in the presence of urea peroxide (Reagent 3b). After stopping the reaction with a strong acid H2SO4 3M, the intensity of the reaction is directly related to the concentration of sCD146 initially contained in the sample.

- 3 x 2 Strips of Reagent 1 (Coated Strip)
- 3 vials of Reagent 2 (Calibrator)
- 6 Tablets of Reagent 3a (ortho-Phenylenediamine)
- 6 Tablets of Reagent 3b (Urea Peroxide)
- 1 vial of Reagent 4 (Dilution Buffer)
- 1 vial of Reagent 5 (Washing Solution)
- 3 vials of Reagent 6 (Anti CD146-Peroxidase)
- 1 vial of Reagent 7 (Control plasma)
- 1 Plate Frame

Sample

Quantity

Assay Range

More information  

Product Name	Ref
Mouse MAb anti-human CD55, clone F4-29D9, FITC conjugated	5129