Presentation
- Stabilization buffer, 10 fold concentrated - Diluent, 10 fold concentrated - Anti human light chains (Kappa + Lambda) - Negative isotypic control - Calibration bead suspension - Staining reagent, polyclonal antibody anti mouse IgG-FITC + platelet counter-staining reagent-PE - 10 GelSep® Tubes (red top) - 10 tubes with filter-cap (blue top)
Principle
Using the GelSep® tube platelets from the sample are separated from the plasma and washed. Then platelets are incubated with a mixture of two monoclonal antibodies specific of Kappa and Lambda human immunoglobulin light chains. After washing a mixture made of a revelation reagent-FITC and a platelet counter-staining reagent-PE is added to the immuno-labelled sample and to a dedicated dual color calibrator
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